Phosphoglucomutases

These enzymes are among the most variable in the P. maniculatus species group. Three reports - Selander, et al. (1971), Mascareflo and Shaw (1973), and Gill (1976) - record natural polymorphisms. Only Gill (1976) conducted breeding studies and established the Pgm-1 and Pgm-4 loci. Selander, et al. (1971) inferred three loci in P. polinotus with 4, 3 and 4 affeles respectively. Mascarello and Shaw (1973) inferred three loci in P. maniculatus with 2, 1 and 3 alleles respectively. In addition to the two loci established by Gill (1976), she inferred two additional loci, one monomeric and one with variation. Locus and allelic designations among these authors do not necessarily correspond.

Phosphoglucomutase-1 (Pgm-1)

ALLELES: Pgm-1^a Pgm-1^b

INHERITANCE: Codominance.

PHENOTYPE: Phenotype is base on electrophoresis of liver homogenate. [Electrophoretic conditions: Gel: Starch, horizontal. Buffer: Continous, phosphate pH 6.7. Visualization: Disodium ?-D-glucose 1,6 diphosphate, dipotassium ?-D-glucose 1,6 diphosphate substrate, with tetrazolium stain.] Zymogram: Mobility: Anodal. Protein structure: Monomer. Electromorphs: Homozygotes show single slow or fast electromorphs, respectively. Heterozygotes possess two electromorphs, one at the fast position and one at the slow position.

SOURCE: Identified from natural polymorphism in P.m. santacnizae, P.m. givnbeli, and P.m. streatoii from Channel Islands and coastal California. Genetic analysis was accomplished at the University of California, Los Angeles, about 1975 using wild - captured and laboratory reared progeny.

REFERENCE: (Genetic analysis): Gill (1976)
(Natural Polymorphism): Selander, et al. (1971) in P. polionotus; Mascarello and Shaw (1973); Gill (1976)

Phosphoglucomutase-4 (Pgm-4)

ALLELES: Pgm-4^a, Pgm-4^b, Pgm-4^c

INHERITANCE: Codominance.

PHENOTYPE: Phenotype is base on electrophoresis of liver homogenate. [Electrophoretic conditions and visulaization: Same as for Pgm-1.] Zymogram: Mobility- Anodal. Protein structure: Monomer. Electromorphs: Homozygotes have a single electromorph in the Pgm-4 position, heterozygotes have two. Which alleles produce fast and slow electromorphs is not stated in the reference.

SOURCE: Identified from natural polymorphism in P.m. clementis, P.m. streatoii, P.m. exterus and P.m. gambeli from Channel Islands and coastal California. Genetic analysis, based on wild - captured animals and their laboratory bred pyrogeny, was accomplished at the University of California, Los Angeles, about 1975.

REFERENCE: (Genetic analysis): Gill (1976). (Natural polymorphism): Selander, et al. (1971) in P. polinotus Mascarello and Shaw (1973); Gill (1976).

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